Safety assessment of Lactobacillus reuteri IDCC 3701 based on phenotypic and genomic analysis

Abstract Purpose Lactobacillus reuteri is one of the most largely studied human-commensal bacteria and widely used as a form probiotics. Safety probiotics has become increasingly important for human consumption due to increasing health-concerns in food industry. In this study, safety L. IDCC 3701 isolated from breast milk was thoroughly investigated. Methods Whole-genome sequence analysis performed identify antibiotic resistance toxigenicity 3701. Phenotypic such minimal inhibitory concentration, β-hemolysis, extracellular enzyme activity, production biological amines L/D-lactate, Finally, acute oral toxicity test access safety. Results Although multiple resistances gentamicin kanamycin were observed 3701, it revealed that these are intrinsic not transferable through whole-genome analysis. addition, various phenotypic concerning hemolysis, D-lactate did show any negative results. harbors histidine decarboxylase gene, no biogenic detected. exhibited evidence according an vivo study. Conclusion Our findings demonstrate considered be safe based on silico, vitro studies.